By Gerard Morel,Annie Cavalier,Lynda Williams
ISBN-10: 0849300444
ISBN-13: 9780849300448
° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
° hybridization ahead of embedding (pre-embedding)
° hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each one strategy, the various levels are defined intimately: the guidance of tissue, pretreatment, hybridization, and visualization of the hybridization items.
The booklet combines concept and perform, beginning with the elemental ideas, then breaking down the experimental method into successive steps illustrated through a number of diagrams, designated protocols, and tables. this can be all performed in a layout that makes use of parallel columns to exhibit worthwhile reviews subsequent to the speculation and functional info along every one level of the protocol. also, the precis tables give you the standards for selecting the probe style and process, and a close index aids within the look for info. in situ Hybridization In Electron Microscopy is an important spouse for utilizing those equipment on the electron microscopic level.
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In Situ Hybridization in Electron Microscopy (Methods in Visualization) by Gerard Morel,Annie Cavalier,Lynda Williams
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